Briefly, primary and secondary antibodies were incubated in pbs with 0. A targeting vector was designed to replace the srybox containing gene 2 sox2 open reading frame with an enhanced green fluorescent protein egfp, a betaactin intron and a rabbit beta. Antibodies research products elisa kits antibodies. Making sense of zebrafish neural development in the minervois.
Written by isabelle nobiron, phd isabelle is a product manager at tebubio, and also the companys iso quality manager. In december 2005, a cancer research team headed by keith cheng at. Sox2 regulates muller glia reprogramming and proliferation in the. Due to a lack of a working antibody against zebrafish nestin and to. Sox2 antibody detects sox2 protein on whole mount zebrafish by immunohistochemical analysis. Jade1 expression was detected in extraembryonic ectoderm and trophoblast, which are placental components important for vasculogenesis, as well as. Mutations in sox2 cause anophthalmiaesophagealgenital aeg. Significant increases in gene expressions of sox2 up to 2fold, sox3 up to 33fold and. Study of pluripotency markers in zebrafish embryos and transient. Zfin provides a wide array of expertly curated, organized and crossreferenced zebrafish research data. The zebrafish epithalamus is part of the diencephalon and encompasses three major. The meeting site was beautifully situated in the heart of the minervois wine country, and its remoteness promoted conversations and interaction over the course of the program. Our sox2 antibodies can be used in a variety of model species. Kirby, in heart development and regeneration, 2010.
Sox2 is required for maintenance and regeneration, but not initial. Optic nerve hypoplasia and abnormalities of the central nervous system. Pdf pleiotropic effects of sox2 during the development of. The sox2 antibody aids brain cancer research the sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. The meeting covered neurogenesis and eye development. Due to their striking genomic similarity to humans, regenerative abilities. Select appropriate sox3 antibodies for your research by isotype, epitope, applications and species reactivity.
Early interest in the cooperative interactions between sox2 and oct4 in esc grew out of a series of studies showing that. Efficient induction of pluripotent stem cells from granulosa cells by oct4 and sox2. The data collected includes not only links to publications in. Neural stem cell marker nestin, sox2, occludin, e cadherin, hes1. Sox10 antibody has been shown to be expressed in 97100% of desmoplastic and spindle cell melanomas and was.
Transcription factor sox an overview sciencedirect topics. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Analysis of nup3 expression in zebrafish embryos by in situ mrna hybridization ish and. Confocal immunofluorescent analysis of ntera2 left, hela center and mouse es cells growing on mef feeder cells right using sox2 l1d6a2 mouse mab green. Sox2 and oct4 levels strongly influence the fate of esc and the formation of ipsc. Select appropriate sox2 antibodies for your research by isotype, epitope, applications and species reactivity. Expression profiling of zebrafish sox9 mutants reveals that. Chicken, feline, fish, human, mouse, porcine, primate, rat, sheep. Sox transcription factors contain an sry dnabinding domain. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press.
These data show that zebrafish sox2 is required for hair cell survival. Select sox3 antibodies from monoclonal antibodies listed below. We at novus biologicals offer a wide variety of highly. Disease ontology term multispecies data omim term omim phenotype id. Characterization of neural stem cells and their progeny in the adult. Sox2 antibody is a mouse monoclonal antibody raised against recombinant human sox2 fragments expressed in e. However, despite all the efforts made to establish zebrafish es cell cultures. Neural stem cell marker nestin, sox2, occludin, e cadherin, hes1, notch1 antibody panel human ab254027 description. The antibody used to label cells expressing sox2 in zebrafish identifies by western blot a main protein band of approximately 34 kda, and an additional small band with an estimated molecular weight of 38. The original zebrafish or zebra danio, danio rerio is a native of rivers in india and bangladesh.
Dako products we help pathologists and clinicians make accurate diagnoses that help determine the most effective treatment for cancer patients we are proud to be a renowned provider of complete. The zebrafish information network zfin is the database of genetic and genomic data for the zebrafish danio rerio as a model organism. Sox2 in taste bud and lateral line system of zebrafish. The antibody used to label cells expressing sox2 in zebrafish identifies by western blot a main protein band of approximately 34 kda, and an additional small band with an estimated molecular weight of 38 kda fig. This antibody recognizes human,mouse,rat, zebrafish antigen. Citeab is a leading life science data provider that helps researchers and their suppliers make more informed decisions. Further analysis of the nestin enhancer revealed an essential sox and. The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the specific staining patterns obtained with particular clones. Apr 04, 2017 citeab is a leading life science data provider that helps researchers and their suppliers make more informed decisions. It is also an important marker in malignant tumors such as melanoma, breast carcinoma, gliomas and benign tumors such as schwannomas.
Our search engine provides a simple way for researchers to find antibodies and other reagents that work, while our high quality life science market data and reagent product citations are used commercially by many of the worlds leading reagent suppliers and investment companies. Neural stem cell marker nestin, sox2, occludin, e cadherin, hes1, notch1 antibody panel human. The sox2 antibody has been validated for the following applications. The sox10 protein is widely expressed in normal human tissues including melanocytes and breast tissue. The sox2 antibody from proteintech is a rabbit polyclonal antibody to a recombinant protein of human sox2. Western blot analysis of extracts from nccit, mouse embryonic stem cells mescs, and f9 cells using sox2 antibody. The sections were then blocked in 1% bsa solution for 10 mins at.
Fluorescence in situ hybridization fish analysis of metaphase chromosomes showed that a bac clone containing sox2, rp1143f17, was deleted in case 1 fig. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Pdf pleiotropic effects of sox2 during the development. Zhiyuan gong and his colleagues at the national university of singapore were working with a gene that encodes the green fluorescent protein gfp, originally extracted from a jellyfish, that naturally produced. Zfin provides a wide array of expertly curated, organized and cross. Several members of the sox family of transcription factors are expressed by neural crest cells and appear to be needed for their induction bell et al. Sox10 protein staining sox10 antibodies biocare medical. In the undamaged adult zebrafish retina, sox2 is expressed in muller glia and a.
Zebrafish antibodies primary antibodies sigmaaldrich. The zebrafish, a small but flashy aquarium pet, may seem like an unlikely informant on questions of human genetics yet its genome could hold the keys to understanding many diseases and. View detailed sox3 antibody specifications by linking to the. Although we tried to test whether sfgfppositive cells express sox2 by immunohistochemistry using two different sox2 antibodies that were previously reported to detect sox2 in zebrafish germana.
In the adult zebrafish cerebellum, sox2 was expressed in purkinje. Gtx124477 ihcwm image sox2 antibody detects sox2 protein on medaka by whole mount immunohistochemical analysis. Pdf developmental changes in the expression of sox2 in the. Expression profiling of zebrafish sox9 mutants reveals. May 01, 2009 expression profiling of zebrafish sox9 mutants reveals that sox9 is required for retinal differentiation hayato yokoi, a, 1 yilin yan, a, 1 michael r.
Previous work has shown that sox2 is expressed in the zebrafish lateral. Early interest in the cooperative interactions between sox2 and oct4 in esc grew out of a series of studies showing that sox2 and oct4 bind cooperatively to two adjacent cisregulatory elements located within a powerful enhancer of the fgf4 gene. Tested in western blot wb, immunofluorescence if, immunocytochemistry icc, immunohistochemistry paraffin ihc p and immunoprecipitation. Anop3,mcops3,mgc24,sox2,sry sex determining region ybox 2,sryrelated hmgbox gene 2,transcription factor sox2,transcription factor sox2, immunogen. The meeting from sensory perception to motor output. Dapi was routinely added to the secondary antibody solution at a.
Srybox containing gene 2 sox2, musashi homolog 1 drosophila msi1. Recently, sox2 antibody preparations identified the sox2 protein as a marker for malignant neural gliomas. Dako products we help pathologists and clinicians make accurate diagnoses that help determine the most effective treatment for cancer patients we are proud to be a renowned provider of complete pathology solutions and flow cytometry reagents, trusted by clinical laboratories around the world in the fight against cancer. The hybridomas are stored at the monoclonal antibody facility of the uo under the supervision of dr. Ab5603, is a highly specific rabbit polyclonal antibody sox2 and has been tested for use in immunocytochemistry, and immunohistochemistry paraffin, and western blotting. Sox2 and sox3 are essential for development and regeneration of. Sox2 is an intronless gene encoding a member of the sryrelated hmgbox sox family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. In addition the study reports experimental and in silico comparative analysis of jade1 mrna transcripts, jade1 gene structure and analysis of jade1 protein orthologues from mouse human and zebra fish. Use the list below to choose the sox2 antibody which is most appropriate for your research. Novel mixfamily homeobox genes in zebrafish and their differential. In teleosts, including zebrafish danio rerio, the entire brain continues. It is also an important marker in malignant tumors such as melanoma, breast carcinoma, gliomas and.
The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval using citric acid ph 6. About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. Pdf developmental changes in the expression of sox2 in. The transcription factor gene sox9 plays various roles in development, including differentiation of the skeleton, gonads, glia, and heart. View detailed sox3 antibody specifications by linking to the specific product blocks. Our search engine provides a simple way for researchers to find. Making sense of zebrafish neural development in the. Find msds or sds, a coa, data sheets and more information. The product of the sox2 gene is required for stemcell maintenance in the central nervous system, and also regulates gene expression in the stomach. The zebrafish blastula embryo consists of the deep embryonic cells of the.
Pleiotropic effects of sox2 during the development of the zebrafish. Thermo fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. Sox2 antibody by reprocell tebubio written by isabelle nobiron, phd isabelle is a product manager at tebubio, and also the companys iso quality manager. Western blot analysis of extracts from nccit and ntera2 cells using sox2 antibody. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding gly179 of human sox2. Maternal nanog is required for zebrafish embryo architecture and for. View detailed sox2 antibody specifications by linking to the specific product blocks. Western blot analysis of extracts from ntera2, f9 and p16 cells using sox2 l1d6a2 mouse mab. Recently, sox2 antibody preparations identified the sox2. Orthologous to human sox2 srybox transcription factor 2. B sox2 morphants have small eyes and short body axis in relation to control siblings. In order to investigate modulation of sox2 expression in spinalcordderived ependymal stemprogenitor cells by connexin 50, abcam sox2 antibody abcam, ab97959 was used in immunohistochemistry frozen section on brown rat samples at 1. View detailed sox2 antibody specifications by linking to the.
It measures three centimeters long and has gold and dark blue stripes. There were significant decreases in sox2 up to 4fold and sox3 up to 3fold expressions following chilling. Abcam antibodies and reagents supplier, find any antibody abcam homepage. The family of sox transcription factors is defined as a family of proteins all of which harbor an hmg box dnabinding domain first identified in the male sex. Anop3,mcops3,mgc24, sox2,sry sex determining region ybox 2,sryrelated hmgbox gene 2,transcription factor sox2,transcription factor sox2, immunogen. On the other hand, sox2 plays an important role in neuronal differentiation, but not in pluripotency. After blocking, the samples were incubated with an antidigoxigenin antibody 1. Select sox2 antibodies from monoclonal antibodies listed below. Jade1 gene structure and analysis of jade1 protein orthologues from mouse human and zebra fish.
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